PABPC1

PABPC1
PDBに登録されている構造
PDB	オルソログ検索: RCSB PDBe PDBj
PDBのIDコード一覧
	1CVJ, 1G9L, 1JGN, 1JH4, 2K8G, 2RQG, 2RQH, 2X04, 3KTP, 3KTR, 3KUI, 3KUJ, 3KUR, 3KUS, 3KUT, 3PKN, 3PTH, 4F02, 4F25, 4F26, 5DX1, 5DXA, 5DX8, 2D9P
識別子
記号	PABPC1, PAB1, PABP, PABP1, PABPC2, PABPL1, poly(A) binding protein cytoplasmic 1
外部ID	OMIM: 604679 MGI: 1349722 HomoloGene: 37638 GeneCards: PABPC1
遺伝子の位置 (ヒト)
染色体	8番染色体 (ヒト)
終点	100,722,809 bp
遺伝子の位置 (マウス)
染色体	15番染色体 (マウス)
終点	36,609,912 bp
遺伝子オントロジー
分子機能	• 血漿タンパク結合; • 核酸結合; • protein C-terminus binding; • translation activator activity; • poly(U) RNA binding; • poly(A) binding; • mRNA 3'-UTR binding; • RNA結合; • mRNA結合;
細胞の構成要素	• 焦点接着; • catalytic step 2 spliceosome; • ストレス顆粒; • 細胞質; • cytoplasmic ribonucleoprotein granule; • spliceosomal complex; • 膜; • エキソソーム; • 細胞核; • 細胞質基質; • 樹状突起; • リボ核タンパク質顆粒; • シナプス; • messenger ribonucleoprotein complex; • リボ核タンパク質;
生物学的プロセス	• ナンセンス変異依存mRNA分解機構; • mRNA stabilization; • mRNA splicing, via spliceosome; • regulation of mRNA stability; • RNAスプライシング; • mRNA polyadenylation; • mRNA processing; • nuclear-transcribed mRNA poly(A) tail shortening; • positive regulation of viral genome replication; • translational initiation; • negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; • positive regulation of translation; • 遺伝子サイレンシング; • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay;
	出典:Amigo / QuickGO
オルソログ
	26986
	18458
	ENSG00000070756
	ENSMUSG00000022283
	P11940,E5RGH3
	P29341
	NM_002568
	NM_008774
	NP_002559
	NP_032800
	ウィキデータ
閲覧/編集ヒト	閲覧/編集マウス

PABPC1（poly(A) binding protein cytoplasmic 1）もしくはPABP1は、ヒトではPABPC1遺伝子にコードされるタンパク質である^[5]。PABPC1はmRNAに結合し、核内外への輸送、分解、翻訳、安定性などさまざまな機能を促進する。ポリ(A)結合タンパク質には主に核内に位置するもの（PABPN1（英語版））と細胞質に位置するもの（PABPC、単にPABPと呼ぶときにはこちらを指すことが多い）が存在し、PABPC1は後者に属する。PABPC1はその位置によって、役割やRNAとの機能が変化する^[6]。

機能[編集]

ポリ(A)結合タンパク質（PABまたはPABP）は真核生物のmRNAの3'末端のポリ(A)テールに複合体を形成しており、ポリ(A)鎖の伸長と翻訳の終結に必要なタンパク質である。PABPC1はPABPファミリーの一部を構成している^[7]。

PABPC1は通常は細胞質内に拡散して存在しており、ストレス顆粒（英語版）、P-body（英語版）、翻訳活性の高い部位など、mRNAが高濃度で存在する部位に濃縮されている。PABPC1はNMDとも関係している。PABPC1はポリ(A)テールやeIF4G（英語版）と相互作用し、環状化したmRNAを安定化している。この構造はNMDを介したmRNA分解を防ぐために必要である^[8]。

PABPC1は核内ではpre-mRNAのポリ(A)テールに結合し、安定性、核外搬出、輸送、分解を促進している。PABPC1は4つのRNA認識モチーフ（RRM）を持つ。最初の2つ（RRM1とRRM2）は、α-インポーチンと、プロセシングされたmRNAの双方に結合する。この結合は、mRNAが核内へ送り返されることを防いでいる^[6]。

相互作用[編集]

PABPC1は次に挙げる因子と相互作用することが示されている。

出典[編集]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000070756 - Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000022283 - Ensembl, May 2017
^ Human PubMed Reference:
^ Mouse PubMed Reference:
^ “Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif”. Nucleic Acids Research 15 (12): 4771–87. (June 1987). doi:10.1093/nar/15.12.4771. PMC 305917. PMID 2885805.
^ ^a ^b “Poly(A)-binding proteins and mRNA localization: who rules the roost?” (英語). Biochemical Society Transactions 43 (6): 1277–84. (December 2015). doi:10.1042/BST20150171. PMID 26614673.
^ “Entrez Gene: PABPC1 poly(A) binding protein, cytoplasmic 1”. 2023年5月5日閲覧。
^ “The interaction of cytoplasmic poly(A)-binding protein with eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA decay” (英語). RNA 20 (10): 1579–92. (October 2014). doi:10.1261/rna.044933.114. PMC 4174440. PMID 25147240.
^ “The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation”. Molecular and Cellular Biology 24 (9): 3577–87. (May 2004). doi:10.1128/mcb.24.9.3577-3587.2004. PMC 387753. PMID 15082755.
^ “Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases”. Genes & Development 21 (23): 3135–48. (December 2007). doi:10.1101/gad.1597707. PMC 2081979. PMID 18056425.
^ ^a ^b “A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation”. The EMBO Journal 17 (24): 7480–9. (December 1998). doi:10.1093/emboj/17.24.7480. PMC 1171091. PMID 9857202.
^ “The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein”. The Journal of Biological Chemistry 274 (24): 16677–80. (June 1999). doi:10.1074/jbc.274.24.16677. PMID 10358005.
^ “Paip1 interacts with poly(A) binding protein through two independent binding motifs”. Molecular and Cellular Biology 22 (11): 3769–82. (June 2002). doi:10.1128/mcb.22.11.3769-3782.2002. PMC 133836. PMID 11997512.
^ “Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation”. Nature 392 (6675): 520–3. (April 1998). Bibcode: 1998Natur.392..520C. doi:10.1038/33198. PMID 9548260.
^ “Dual interactions of the translational repressor Paip2 with poly(A) binding protein”. Molecular and Cellular Biology 21 (15): 5200–13. (August 2001). doi:10.1128/MCB.21.15.5200-5213.2001. PMC 87244. PMID 11438674.

関連文献[編集]

“The mRNA poly(A)-binding protein: localization, abundance, and RNA-binding specificity”. Experimental Cell Research 211 (2): 400–7. (April 1994). doi:10.1006/excr.1994.1104. PMID 7908267.
“Human immunodeficiency virus type 1 Rev is required in vivo for binding of poly(A)-binding protein to Rev-dependent RNAs”. Journal of Virology 68 (9): 5433–8. (September 1994). doi:10.1128/jvi.68.9.5433-5438.1994. PMC 236943. PMID 8057425.
“Role of polyadenylation in nucleocytoplasmic transport of mRNA”. Molecular and Cellular Biology 16 (4): 1534–42. (April 1996). doi:10.1128/mcb.16.4.1534. PMC 231138. PMID 8657127.
“Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation”. Nature 392 (6675): 520–3. (April 1998). Bibcode: 1998Natur.392..520C. doi:10.1038/33198. PMID 9548260.
“The human poly(A)-binding protein 1 shuttles between the nucleus and the cytoplasm”. The Journal of Biological Chemistry 273 (21): 13015–21. (May 1998). doi:10.1074/jbc.273.21.13015. PMID 9582337.
“A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation”. The EMBO Journal 17 (24): 7480–9. (December 1998). doi:10.1093/emboj/17.24.7480. PMC 1171091. PMID 9857202.
“The expression of poly(A)-binding protein gene is translationally regulated in a growth-dependent fashion through a 5'-terminal oligopyrimidine tract motif”. The Journal of Biological Chemistry 274 (3): 1708–14. (January 1999). doi:10.1074/jbc.274.3.1708. PMID 9880551.
“Control of mRNA decay by heat shock-ubiquitin-proteasome pathway”. Science 284 (5413): 499–502. (April 1999). Bibcode: 1999Sci...284..499L. doi:10.1126/science.284.5413.499. PMID 10205060.
“The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein”. The Journal of Biological Chemistry 274 (24): 16677–80. (June 1999). doi:10.1074/jbc.274.24.16677. PMID 10358005.
“Recognition of polyadenylate RNA by the poly(A)-binding protein”. Cell 98 (6): 835–45. (September 1999). doi:10.1016/S0092-8674(00)81517-2. PMID 10499800.
“Chromosomal localization of three human poly(A)-binding protein genes and four related pseudogenes”. Human Genetics 105 (4): 347–53. (October 1999). doi:10.1007/s004390051113. PMC 1865476. PMID 10543404.
“A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex”. Cell 103 (1): 29–40. (September 2000). doi:10.1016/S0092-8674(00)00102-1. PMID 11051545.
“Translational repression by a novel partner of human poly(A) binding protein, Paip2”. Molecular Cell 7 (1): 205–16. (January 2001). doi:10.1016/S1097-2765(01)00168-X. PMID 11172725.
“Disruption of the interaction of mammalian protein synthesis eukaryotic initiation factor 4B with the poly(A)-binding protein by caspase- and viral protease-mediated cleavages”. The Journal of Biological Chemistry 276 (26): 23922–8. (June 2001). doi:10.1074/jbc.M100384200. PMID 11274152.
“Human testis expresses a specific poly(A)-binding protein”. Nucleic Acids Research 29 (9): 1872–83. (May 2001). doi:10.1093/nar/29.9.1872. PMC 37253. PMID 11328870.
“Dual interactions of the translational repressor Paip2 with poly(A) binding protein”. Molecular and Cellular Biology 21 (15): 5200–13. (August 2001). doi:10.1128/MCB.21.15.5200-5213.2001. PMC 87244. PMID 11438674.
“Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells”. The Journal of Biological Chemistry 277 (8): 6428–37. (February 2002). doi:10.1074/jbc.M109446200. PMID 11704675.
“PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays”. EMBO Reports 3 (3): 268–73. (March 2002). doi:10.1093/embo-reports/kvf052. PMC 1084016. PMID 11850402.
“Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis”. RNA 8 (4): 426–39. (April 2002). doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000070756 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000022283 - Ensembl, May 2017

[3] Human PubMed Reference:

[4] Mouse PubMed Reference:

[pmid2885805-5] “Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif”. Nucleic Acids Research 15 (12): 4771–87. (June 1987). doi:10.1093/nar/15.12.4771. PMC 305917. PMID 2885805.

[Gray_2015-6] “Poly(A)-binding proteins and mRNA localization: who rules the roost?” (英語). Biochemical Society Transactions 43 (6): 1277–84. (December 2015). doi:10.1042/BST20150171. PMID 26614673.

[entrez-7] “Entrez Gene: PABPC1 poly(A) binding protein, cytoplasmic 1”. 2023年5月5日閲覧。

[:0-8] “The interaction of cytoplasmic poly(A)-binding protein with eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA decay” (英語). RNA 20 (10): 1579–92. (October 2014). doi:10.1261/rna.044933.114. PMC 4174440. PMID 25147240.

[pmid15082755-9] “The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation”. Molecular and Cellular Biology 24 (9): 3577–87. (May 2004). doi:10.1128/mcb.24.9.3577-3587.2004. PMC 387753. PMID 15082755.

[pmid18056425-10] “Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases”. Genes & Development 21 (23): 3135–48. (December 2007). doi:10.1101/gad.1597707. PMC 2081979. PMID 18056425.

[pmid9857202-11] “A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation”. The EMBO Journal 17 (24): 7480–9. (December 1998). doi:10.1093/emboj/17.24.7480. PMC 1171091. PMID 9857202.

[pmid10358005-12] “The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association of erf3/GSPT with polyadenylate-binding protein”. The Journal of Biological Chemistry 274 (24): 16677–80. (June 1999). doi:10.1074/jbc.274.24.16677. PMID 10358005.

[pmid11997512-13] “Paip1 interacts with poly(A) binding protein through two independent binding motifs”. Molecular and Cellular Biology 22 (11): 3769–82. (June 2002). doi:10.1128/mcb.22.11.3769-3782.2002. PMC 133836. PMID 11997512.

[pmid9548260-14] “Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation”. Nature 392 (6675): 520–3. (April 1998). Bibcode: 1998Natur.392..520C. doi:10.1038/33198. PMID 9548260.

[pmid11438674-15] “Dual interactions of the translational repressor Paip2 with poly(A) binding protein”. Molecular and Cellular Biology 21 (15): 5200–13. (August 2001). doi:10.1128/MCB.21.15.5200-5213.2001. PMC 87244. PMID 11438674.

[1]

[2]

[3]

[4]

[5]

[6]

[7]

[8]

[9]

[10]

[11]

[12]

[13]

[14]

[15]