解説Viral luciferase expression in a mouse tumour.jpg
English: A 'nude' (hairless) mouse bearing a tumour in the right hind leg has been treated intra-tumourally with vaccinia virus encoding luciferase. Viral infection of the tumour is visible by the bio-luminescence produced by luciferase. (False coloring is used to demonstrate intensity). Citation: Haddad D, Chen C-H, Carlin S, Silberhumer G, Chen NG, et al. (2012) Imaging Characteristics, Tissue Distribution, and Spread of a Novel Oncolytic Vaccinia Virus Carrying the Human Sodium Iodide Symporter. PLoS ONE 7(8): e41647. doi:10.1371/journal.pone.0041647
{{subst:Upload marker added by en.wp UW}} {{Information |Description = {{en|A 'nude' (hairless) mouse bearing a tumour in the right hind leg has been treated intra-tumourally with vaccinia virus encoding luciferase. Viral infection of the tumour is vis...
<title>Optical and histologic detection of viral replication using vaccinia marker genes.</title><p>A. Presence of GLV-1h153 in tumors was detected histologically, shown here with the IT group 2 days post virus injection. Areas staining positive with antibodies against GFP and VACV antigen A27L corresponded and were easily visualized in GLV-1h153-injected tumors, whereas no staining was evident in untreated tumors. Areas of A27L staining is also shown at 400× magnification. B. GFP expression was optically monitored, shown here 5 weeks postinjection. Control tumors were larger than GLV-1h153-treated tumors, both when administered intratumorally or intravenously. GLV-1h153 was also detected via bioluminescence imaging 2 weeks posttreatment.</p>